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81.
82.
采用等电聚焦电泳技术对适于西北地区的几种冷季型草坪草种进行了品种真实性鉴定研究,并使用国际认证的计算机软件(ImageMaster1D)进行同工酶谱带分析,对检测结果进行了定性定量评定,充分表明了品种间基因多态性,证明此方法是草坪草品种鉴别的有效测定技术。 相似文献
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84.
Effects of method, rate and timing of slurry application to grassland on the preference by cattle for treated and untreated areas of pasture 总被引:1,自引:0,他引:1
Two experiments were conducted in the UK to investigate preference by cattle for areas of pasture treated or untreated with dairy cow slurry. Experiment 1 examined the effects of method and rate of slurry applied to a grass pasture in March (Spring); Experiment 2 examined the effects of method and timing of slurry application after cutting for silage in June (Summer). In both experiments, beef steers grazed grassland plots that had been treated with slurry on one half only of the plots for 4 weeks. Slurry was applied by shallow injection (I), trailing shoe (T) or conventional surface broadcasting (S) techniques. In Experiment 1, slurry was applied at 40 m3 ha?1 (HI) or 20 m3 ha?1 (LO) 21 d before grazing commenced; in Experiment 2, slurry was applied at 20 m3 ha?1 either 32 d (E) or 10 d (L) before grazing commenced. Sward height was measured and animal behaviour was recorded during 8 × 24 h measurement periods in each experiment. Preference for slurry‐treated or untreated swards was indicated by a comparison of post‐grazing sward height on each sward and the proportion of total grazing time spent on the slurry‐treated swards. In Experiment 1, a smaller proportion of grazing time was spent on slurry‐treated areas of swards compared with untreated areas of swards on all treatments, but the proportion of time spent grazing the slurry‐treated sward was greater on method T than on methods I or S (0·28, 0·31 and 0·39 for methods S, I and T, respectively, s.e.d. = 0·035, P < 0·05). Steers showed no preference between slurry‐treated and untreated swards by 49, 45 and 44 d after slurry application for methods S, I and T respectively. Differences between HI and LO were not significant (P > 0·05). In Experiment 2, aversion to slurry‐treated swards compared with untreated swards was less for treatment E than for L, with a greater proportion of both residence and grazing time spent on the slurry‐treated sward of treatment E than L (0·43 and 0·28, respectively, for residence time, s.e.d. = 0·025, P < 0·001, and 0·47 and 0·30, respectively, for grazing time, s.e.d. = 0·021, P < 0·001). Significant differences were shown between the methods of slurry application for treatment E, with a greater proportion of grazing time spent on the slurry‐treated swards of methods I and T than method S (0·40, 0·52 and 0·49 for S, I and T, respectively, s.e.d. = 0·032, P < 0·001), and a greater difference between post‐grazing heights on slurry‐treated and untreated swards for method S than for I or T (30, 12 and 15 mm, respectively, s.e.d. = 4·8, P < 0·001). For treatment L, differences in the proportion of time spent grazing on slurry‐treated swards compared with untreated swards were not significant between treatments (0·26, 0·34 and 0·31 for methods S, I and T, respectively, s.e.d. = 0·040, P > 0·05) and indifference between slurry‐treated and untreated swards was achieved 77, 60 and 58 d after slurry spreading for methods S, I and T respectively. These results show that, when slurry is applied to pasture in a grazing rotation, the adverse effects on grazing behaviour associated with conventional surface broadcasting may be overcome by the use of novel slurry application techniques. 相似文献
85.
利用加入氨甲喋呤和胸苷使细胞分裂同步化并结合胰酶G带技术,分析了猪前中期染色体高分辨G带。单套染色体的G带数目,包括x和y染色体,前中期为503条,中期为300条。绘制了前中期和中期G带带型图。 相似文献
86.
苎麻染色体核型和Giemsa C-带型及PMC减数分裂行为的研究 总被引:1,自引:0,他引:1
本试验以苎麻品种芦竹青为材料,从有丝分裂和减数分裂两个方面进行研究,有丝分裂以嫩稍扦插诱导萌发幼根,采用F-BSG法制备染色体标本,对其染色体核型和Giemsa带型进行的分析结果表明,苎麻的核型公式为2n=28=8(L)st+18(S)st+2(S)SAT,全为不对称的染色体,N·F,值与其染色体的条数相等,每组染色体的臂指数均大于78%,染色体长度比大于2.1:1,属于4B类核型,Giemsa C-带带型单一,短臂为全带,长臂为着丝点带,减数分裂以2%醋酸洋红压片,发现同一株上幼蕾着生的部位不同,其发育进度不一致,以着生在茎的中,上部的幼蕾发育快,同一枝梗上以着生于下部的幼蕾发育早,而同一幼蕾的PMC在减数分裂终变期,后期I,末期I以及后期I,四分体等阶段同步程度高,同时观察到存在一定的高峰期。 相似文献
87.
本试验较系统地研究了黄花菜根尖细胞染色体的核型特征和带型特征,其中,对黄花菜G—带带型的研究,为国内首次报道。 相似文献
88.
Carrillo JM Sopena JJ Rubio M Redondo JI Serra I Soler C 《Veterinary surgery : VS》2005,34(4):387-392
OBJECTIVE: To evaluate the use of polyamide bands, manufactured for securing electrical cables, for repair of oblique femoral fractures in rabbits. STUDY DESIGN: Experimental study. ANIMALS: Twelve nulliparous, 21-25-week-old, California female rabbits. METHODS: Rabbits were divided into 3 groups (n=4) and studied for 14, 28, or 56 days. A Z-shaped mid-diaphyseal femoral osteotomy was repaired with a 2.5 mm intramedullary pin and a polyamide 6.6 cerclage band. Healing was evaluated at intervals by physical examination, limb use, femoral radiographs, and callus histology. RESULTS: Rabbits had early limb use with good wound healing. From the 1st day, movement of the hip and stifle joints was satisfactory. Radiographically, fractures were healed at 28 days. Histologically, there was no foreign body reaction and bone healing was normal. CONCLUSION: Nylon cerclage band application was accomplished easily, maintained reduction, and resulted in good healing and limb use. CLINICAL RELEVANCE: Sterilized nylon bands, manufactured for electrical use, can be used for cerclage in rabbits. 相似文献
89.
天山地形对新疆大风和降温天气作用的数值模拟研究 总被引:2,自引:0,他引:2
2009年5月24日夜间~26日夜间,新疆地区自西向东出现了明显的降水、大风、降温天气过程。为研究此次天气过程中天山地形的作用,文中用WRFV3.1模式对其进行了数值模拟,并通过改变天山山脉的地形高度设计了一组敏感性试验,分析了天山地形对此次强天气过程中大风和降温的影响。结果表明,天山山脉地形对准格尔盆地西北部直至天山山脉北侧的气流有明显的阻挡与减缓作用,对三十里风区、百里风区、汗腾格里峰北坡强风带的形成有直接而重要的作用;地形高度越高,产生垂直运动的地形抬升机制越强,地形强迫抬升作用产生的垂直上升运动越剧烈;天山山脉明显地减轻了寒潮天气过程中南疆塔里木盆地的降温幅度,对准格尔盆地北部的寒潮降温幅度也有一定的减缓作用,对塔里木盆地的"保温"作用较准格尔盆地明显。 相似文献
90.